Molecular weight 407.99. The structural formula of its chloride is: Once it is dissolved, dilute to 100.00 ml with acetic acid. The use of gentian violet (crystal violet) as a bacterial stain led, eventually, to its use as an antibacterial. CAS RN. Crystal violet (Basic Violet 3) is a triarylmethane dye. Riboflavin, also known as vitamin B 2, is non-toxic and an essential vitamin required for the functions of the human body. Odor Threshold N/A. Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. * "" means soluble, but saturation unknown. Crystal violet is a triarylmethane dye. Crystal violet Cell Culture Tested Product Code: TC510 Disclaimer : User must ensure suitability of the product(s) in their application prior to use. Crystal violet is a triarylmethane dye. Reply:try chloroform or lots of hot water. 6.3. Conclusions. Partially miscible with alcohol and glycerol. Conditions to Avoid: Incompatible materials, ignition sources . It is a component of gram staining in microorganisms and cell lines. Reagent and Dye Solubility Chart Reagent and Dye Solubility Chart Roy C. Ellis IMVS Division of Pathology The Queen Elizabeth Hospital Woodville Road, Woodville, South Australia 5011 Email: [email protected] This is to be used as a guide only as solubility data varies between manufacturers for the same product especially for dyes)1-3 . -Crystal Violet is a non-toxic alternative, used in place of the fluorescent dye ethidium bromide in DNA gel electrophoretic runs. Odor No odor. Targets Solubility * Preparing Stock Solutions * The above data is based on the productmolecular weight 407.98. Get All The Latest News Help & Support Center English English; Change Country; 0 . Prepare Crystal Violet Stain. How do I clean crystal violet from a beaker? 42555) CAS 548-62-9 indicator ACS,Reag. Crystal violet (C.I. The product contains volatile substances which may spread in the atmosphere. Crystal violet or methyl violet is used in many applications, including: As a pH indicator (yellow to violet with the transition at a pH = 1.6) In the medical community, it is the active ingredient in Gram's Stain, used to classify bacteria. No. Remove contact lenses, if present and easy to do. PubChem Substance ID. Add 0.02 g of crystal violet in 75 ml of DI water. 30.54/Pack Qty. Methods and material for containment and cleaning up Preparing Stock Solutions. Key features and details. It is used as a pH indicator, with a range between 0 and 1.6. Question: Why are serial dilutions required, and not just a simple dilution for visible spectrophotometry? If eye irritation persists: Get medical advice/attention. MSDS # 237.00 Crystal Violet Solution, 0.1% Scholar Chemistry Section 9: Physical and Chemical Properties Molecular formula C 25 H 30 N 3 Cl. Vapor Density (air=1) 0.7 (water). Cite this Article. Gram staining involves three processes: staining with a water-soluble dye called crystal violet, decolorization, and counterstaining, usually with safanin. The colour of the dye depends on the acidity of the solution. This problem has been solved! It is used in textile and paper dye industries as well as navy blue and black inks for printing, ball-point pens and inkjet printers. Stability and Reactivity Reactivity: Chemical Stability: Stable Conditions of Stability/Instability: N/A Source: Synthetic. Chemical Name CAS Number 1DPH 54 CERCLA RQ 734 CAA 112(2) TQ Crystal Violet 548-62-9 No No No No No Section 16 Additional Information Revised: 09/03/2014 Replaces: 09/03/2014 Printed: 04-21-2015 Products conform solely to the information contained in this and other . Other solubilities: soluble in chloroform, 1 g/10 mL alcohol, 1 g/15 mL glycerin, practically . Crystal Violet (Gentian Violet) has antiviral effects against H1N1 and also has prominent bactericidal activities. Solubility ethanol Absorption maximum Colour Empirical formula Formula weight: Crystal violet Crystal violet Gentian violet Methyl violet 10B 42555 Basic violet 3 Triarylmethane Basic 1.68% 13.87% 593 (Gurr), 588 (Aldrich) Blue violet C 25 H 30 N 3 Cl 408: Crystal violet is the deepest blue of all the methyl violets, and is satisfactory for . An intense purple coloration results. Posted by Barbosa at 10:47 PM. Contains: Crystal Violet 0.1% (CAS 548-62-9), .05% ethyl alcohol (CAS 64-17-5), 99.95% water (CAS 7732-18-15) Weber Scientific 2732 Kuser Road | Hamilton, NJ . Cation-based violet dye. For use as a stain for bacteria, Add 2 g of crystal violet to 20 ml of 95% ethyl alcohol. Due to differences in the thickness of a peptidoglycan layer in the cell membrane between Gram positive and Gram negative bacteria, Gram positive bacteria (with a thicker peptidoglycan layer . Leucocrystal Violet has been reported that leucocrystal violet can be oxidized with acidified potassium iodate forming crystal violet dye, which shows a maximum absorbance at 590 nm. Insoluble in ether. Product description: Crystal Violet is used as an acid-base indicator. Draw the mechanism of formation of crystal violet starting with the formation of the Grignard reagent. If using pH 7.0 water fails to improve solubility, I would suggest dissolving the Crystal violet first in 0.25% methanol, and then diluting this in water to a working concentration of 0.05%. crystal violet,physical properties,suppliers,CAS,MSDS,structure,Molecular Formula, Molecular Weight ,Solubility,boiling point, melting point . Solubility: 10 g/l: Dimensions; Materials Information; Toxicological Information; LD 50 oral: LD50 Rat 420 mg/kg: Safety Information according to GHS; When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590 nm and an extinction coefficient of 87,000 M 1 cm 1 . The protonated form (found in acidic conditions) is yellow, turning blue-violet above . water-soluble and may spread in water systems. 2. Crystal Violet Dye content 90 %; CAS Number: 548-62-9; EC Number: 208-953-6; Synonyms: Basic Violet 3,Gentian Violet,Hexamethylpararosaniline chloride,Methyl Violet 10B; Linear Formula: C25H30N3Cl; find Sigma-Aldrich-C3886 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich * In solvent : -80C, 6 months; -20C, 1 month (protect from light) Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol. Why would water be a strong enough acid to eliminate the hydroxy in this . Science; Chemistry; Chemistry questions and answers; How does the low pH affect the solubility of the crystal violet? Thermo Scientific Crystal Violet, certified . Gentian violet | C25H30ClN3 | CID 11057 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities . Related Products: Crystal Violet. Continue rinsing. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Crystal Violet 548-62-9 Trade Secret Water 7732-18-5 Trade Secret Ammonium Oxalate 6009-70-7 Trade Secret 4. From these solubility figures it is evident that it would be impossible to make an aqueous solution of gentian violet in a concen tration of more than about 3 per cent. Waste water containing CV may affect aquatic ecosystems. When dissolved in water, the dye has a blue-violet colour with an absorbance maximum at 590nm and an extinction coefficient of 87,000 M-1 cm-1. CAS Number: 548-62-9. Solubility Soluble in water. Crystal Violet Solubility: Soluble in water (50 mg/ml at 27 C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Identification Product Name: Crystal Violet, 1% Item #: MA0103018, SKC1012-250, SKC1012-500 Web SDS: S213 . Its solubility in water is 1.68% and its solubility in 95% ethanol is 13.87%. Crystal Violet BP is an organic compound with antifungal and antibacterial capabilities. Art. Seal the absorbent paper, and any of your clothes, which may be contaminated, in a vapor-tight plastic bag for eventual disposal. In addition to the stain itself, the solution generally contains phenol to aid the absorption of the stain by the sebaceous material in the fingermark. Toxicity : rats and mice by mouth LD 50 were 1.0 and 1.2g/kg. Leucocrystal violet is a triphenylmethane dye which can be used to detect antimony in environmental and biological samples using spectrophotometric techniques. : 785 42555. form powder Batteries; Burners; Clipboards; Labeling; Laboratory Supplies, Miscellaneous; Magnifiers; Markers; . The compound helps to access bacterial contamination of tissue culture samples. Crystal violet lactone MSDS(Chinese) Safety Information. Both its aqueous solution and alcohol solution are purple. Why is crystal violet soluble? Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water. 1552-42-7. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with . Newer Post Older Post Home. Melting . 4. Compare this item. It dissociates into positive and negative ions that can penetrate bacterial cells. Chemsrc provides Crystal violet lactone(CAS#:1552-42-7) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Not for Human or Veterinary or Therapeutic Use. Description. Notes No. Its photodynamic action is elicited via a free-radical mechanism. Ph Eur - Find MSDS or SDS, a COA, data sheets and more information. ?s method of classifying bacteria. . Dilute to a final volume of 100 ml with DI water. Crystal/gentian violet. VAT. Crystal Violet assay protocol summary: - remove culture medium - wash cells - add Crystal Violet staining solution . The product contains volatile substances which may spread in the atmosphere. How can the solubility of crystal violet be increase to clean the glassware? T123.1 In stock Delivery fast, simple and reliable! . Last Update2022-01-01 08:49:51. crystal violet o - Nature. One of the key events during the very early development of the new aniline dyes as biological stains was the introduction of the Gram stain (see Section 6.1.2). 58 / Monday, March 26, 2012 / Rules and Regulations Date of issue: 12/30/2014 Revision date: 05/03/2017 Supersedes: 09/14/2015 Version: 1.2 05/03/2017 EN (English US) Page 1 SECTION 1: Identification 1.1. Appearance Dark blue-violet liquid. . Therefore, this method can realize sensitive detection of crystal violet. Subscribe to: Post Comments (Atom) Crystal violet (CV) dye is triphenylmethane cationic dye (figure 1). Crystal Violet (Ammonium Oxalate) According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 . No comments: Post a Comment. Solubility: Solubility in water: 16g/L (25C). Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines. This paper investigated the preparation of CDs with 1,2,4,5-tetraaminobenzene as the raw material. 42555 Shelf Life: 36 Months. It is mostly utilized as textile dye and paper dye and It can be used to treat itching in fish because of its antimicrobial properties. Leucocrystal Violet | C25H31N3 | CID 69048 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities . How to prepare crystal violet indicator for titration: Weigh accurately 0.5 gm of crystal violet and pour it into a 100.00 ml volumetric flask containing 50.00 ml of acetic acid and swirl until it completely dissolved. Riboflavin is photosensitive to UV and visible light in terms of . Crystal violet (CV) is applied in daily use mainly as a commercial dye and antimicrobial agent. Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. Splash some acetone in. C26H29N3O2 = 415.54. Water Solubility: 16 g/L (25) Physical and Chemical Properties: melting point 215C water-soluble 16g/L (25C) Use: Used as a biological stain: . crystal violet dye solvent solubility double salt Prior art date 1945-07-20 Legal status (The legal status is an assumption and is not a legal conclusion. | 25 g per Pack Qty. The emission wavelength is adjustable merely through the control of the solvent. Concentration: 0.2% w/v in Glacial Acetic Acid Composition: Acetic Acid 99.81%, Gentian Violet 0.19% Boiling Point: Approximately 118C Density: 1.05 Melting Point: 16.64C Color: Dark blue to violet/purple liquid Physical State: Liquid Flash Point: 39C pH Range: Solubility. Crystal Violet Biological Activity. Leucocrystal Violet is a triphenylmethane dye used in the detection of antimony in environmental and biological samples using spectrophotometric techniques. It has been used in creams for the topical treatment of bacterial and fungal infections, being effective against some Gram-positive bacteria (notably Staphylococcus species) and some pathogenic fungi (including Candida species) but use declined following reports of animal carcinogenicity. All products from TargetMol are for Research Use Only. Product details 32.15 /Pack Qty. Basically you need to workout what it's soluable in and use that solvent. At a pH of >1.0, the dye is green with absorption maxima at 420nm and . Gentian violet was first produced in the 19th century and was used for some time as an antibiotic and antifungal. Crystal Violet has antibacterial and antifungal properties. Solubility: Alcohol and Water Synonyms: Gentian Violet, Basic Violet 3, C.I. The dye destroys cells and is used as a moderate-strength external disinfectant. However, the solubility of its iodide in two solvents is only 0.035% and 1.78% respectively. Shop Thermo Scientific Crystal Violet, pure, indicator at Fishersci.co.uk English English; Change Country. Description. Gentian violet, also known as crystal violet and methyl violet 10B, is a blue-violet dye. Reaxys Registry Number. Crystal violet (C.I. Expired - Lifetime Application number US606284A Inventor Herrick Guy Scott AC447570500 $57.60 / Each of 1 Order in Bulk Request bulk or custom formats Qty Check Availability Add to cart Description Specifications Safety and Handling Description A staining solution containing gentian violet ( crystal violet) may be employed for the detection of the fatty components in the latent fingerprint deposit. It is known in medicine as Gentian violet (or crystal violet or pyoctanin (e) [2]) and is the active ingredient in a Gram stain, used to classify bacteria. Generally, it requires a strong acid such as sulfuric acid eliminate a hydroxy group. Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. Filter the stain if necessary. Crystal violet, also called gentian violet, is soluble in water and alcohol and is used for multiple histological stains, e.g. Insoluble . Target: Others Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. . Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei. Crystal Violet Page 4 of 4 Section 15 Regulatory Information TSCA Status: All components in this product are on the TSCA Inventory. Physical State (20 deg.C) Solid. How can the solubility of crystal violet be increase to clean the glassware? Besides, crystal violet also shows good solubility in ethanol. Chemistry questions and answers. Crystal Violet, certified by the Biological Stain Commission C6158 Crystal Violet, ACS reagent, 90.0% anhydrous basis 61135 Crystal Violet, for microscopy (Bact., Bot., Hist., Vit. which are water-soluble and may spread in water systems. ), indicator (pH 0.1-2.0) 46364 Crystal Violet, VETRANAL, analytical standard 32675 Crystal Violet, indicator for the determination of the redox potential, S. Gram staining. Identification Solubility: Solubility in water: 16g/L (25C). Crystal violet is insoluble in ether, it is soluble in water, chloroform and alcohol. 42555) for microscopy Certistain, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. Molecular Formula / Molecular Weight. Solubility Miscible with water and chloroform. Related Products: Crystal Violet Stain. Crystal Violet Preparation For Cell Staining: For crystal violet solution you dilute 0.125g of crystal violet in 50 ml of 20% methanol. Thermo Scientific Crystal violet, ACS reagent Manufacturer: Thermo Scientific 447570500 View more versions of this product Catalog No. Product Application: Crystal Violet is the primary stain used in the Gram stain technique for differentiation of Gram-negative versus Gram-postive bacteria. Question: How does the low pH affect the solubility of the crystal violet? The colour of the dye depends on the acidity of the solution. The concentration of the prepared solution is about 05.00 gram/liter. The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead". from 6 Pack Qty. However, physicians prescribe solutions of the dye in con Solubility 20 ppm in water yields clear dark purple solution Absorption maxima 580.00 -595.00 Loss on drying (at 110C . in 30 to 40 cc. Articles of Crystal violet lactone are included as well. Methyl violet 10B has six methyl groups. It is soluble in water, alcohol and chloroform, but insoluble in ether. Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. Form / State: Solid. 28.94/Pack Qty. 344787. Soluble in alcohol, chloroform-soluble, water-soluble, non-soluble ether. Solubility Testing; Filter Papers; Laboratory Seating; Laboratory Supplies, General. Synonyms: Hexamethylpararosaniline Chloride; Gentian Violet; Basic Violet 3; Methyl Violet 10B; Pyoctanin; C.I. The compound helps to assess bacterial contamination of tissue culture samples. from 24 Pack Qty. It is also used to colourize diverse products such as fertilizers, antifreeze, detergents and leather. It's derived from coal tar and is presently used to treat thrush (a fungal infection) of the mouth and skin [1, 2]. 77, No. No. but is now mostly regarded as a synonym for crystal violet. of water. SECTION 1: Identification of the substance/mixture and of the company/undertaking . The dye is used as a histological stain and in Gram? Specific Gravity 1.00 g/mL @ 20C. Solubility: N/A Decomposition Temperature: N/A 10. BuyersGuideChem provides reliable information about Crystal violet (548-62-9) like chemical properties, structure, melting point density, molecular formula, molecular weight, physical properties, toxicity information. Use absorbent paper dampened with 60-70% ethanol to pick up any remaining material. Compare this item. It has been used in creams for the topical treatment of bacterial and fungal infections, being effective against some Gram-positive bacteria (notably Staphylococcus species) and some pathogenic fungi (including Candida species) but use declined following reports of animal carcinogenicity. Molecular Weight: No information found Section 10 - Stability and Reactivity Chemical Stability: Stable under normal temperatures and pressures. Water Solubility: 0.1 g/100 mL at 22.5 C MSDS. A component used in gram staining as a counter-stain to Safranin O (Prod # S7400). Crystal Violet, CAS 548-62-9 Bulk Inquiry Add to Cart Select Batch Purity 100.00% LCMS COA Datasheet SDS Used as a component of CV cytotoxicity assays. Symbol: GHS07: Signal Word: Warning . CRYSTAL VIOLET TS C900.7E INTEGRA Chemical Company24 Hour Emergency Response: CHEMTREC 800-424-9300 (Outside USA 703-527-3887) Concentration: 0.2% w/v in Glacial Acetic Acid Composition: Acetic Acid 99.81%, Gentian Violet 0.19% Boiling Point: Approximately 118C Density: 1.05 Melting Point: 16.64C Color: Dark blue to violet/purple liquid Physical State: Liquid Flash Point: 39C pH Range: Solubility. gives the solubility of the dye as 1 Gm. . Crystal violet is a triarylmethane dye that is a mixture of violet rosanilinis, with antibacterial, antifungal, and anthelmintic properties. Crystal Violet, 1% 1. Crystal Violet According to Regulation (EC) No 1907/2006, Annex II, as amended. Crystal Violet, 1% w/v in Glacial Acetic Acid Safety Data Sheet according to Federal Register / Vol. Soluble in water 50 g / L at 27C. . excl. . Crystal Violet. Specific Gravity/Density: 1 (Water=1) Molecular Formula: Not applicable. Soluble in water to 25 mM, in DMSO to 100 mM and in ethanol to 10 mM. Other solubilities: soluble in chloroform, 1 g/10 mL alcohol, 1 g/15 mL glycerin, practically insoluble in ether: Color: Green: CAS: 548-62-9,90-94-8: Dye Content: 88% min. Room temperature in continental US; may vary elsewhere. Since the crystallization of water are nine elements crystal water. Sparingly Soluble in Water, Freely Soluble in Ethanol (96%) and In Methylene Chloride: Passes Test: 3: Identification . The compound helps to access bacterial contamination of tissue culture samples. This aniline-derived dye has various antimitotic and antifungal properties. Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. Crystal Violet Solution Version: 18 Solubility in water: Soluble. Soluble in water (50 mg/ml at 27 C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Grignard Reaction to form Crystal Violet Lab 1. Solvent wash all contaminated surfaces with 60-70% ethanol followed by washing with a soap and water solution. Concentration Solvent Mass 1 mg 5 mg 10 mg; 1 mM: 2.4511 mL: 12.2555 mL: 24.5110 mL: 5 mM: 0. . Crystal Violet is used as an acid-base indicator, alcohol denaturant, biological stain, textile dye, and an indicator for copper salts. First Aid Measures Eye Contact: If in eyes: Rinse cautiously with water for several minutes. Crystal violet is a blue monochloride salt of crystal violet cation. For lysing solution, dilute 5.88 g of sodium . Crystal violet has antibacterial, antifungal, and anthelmintic ( vermicide) properties and was formerly important as a topical antiseptic. When the purple crystal is dissolved in water, it will turn blue or purple.